Histone 3 anticorps (H3K9ac, H3K14ac)

N° du produit ABIN5706782

N° du produit (Fournisseur): 600-401-i73

L’anticorps Lapin Polyclonal anti-Histone 3 a été validé pour WB, IHC, IF, ChIP, DB, FM et MA. Il convient pour détecter Histone 3 dans des échantillons de Humain, Souris et C. elegans.

Aperçu rapide pour Histone 3 anticorps (H3K9ac, H3K14ac) (ABIN5706782)

Antigène: Histone 3 (H3) (Histone H3 (H3))

Reactivité: Humain, Souris, C. elegans

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Histone 3 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP), Dot Blot (DB), Fluorescence Microscopy (FM), Multiplex Assay (MA)

Détail du produit anti-Histone 3 anticorps

Épitope: H3K9ac, H3K14ac

N° du produit (Fournisseur): 600-401-i73

Fournisseur: Rockland

Fonction: Histone H3 K9ac/K14ac Antibody

Réactivité croisée (Details): This antibody reacts with human Histone H3.

Attributs du produit: Synonyms: rabbit anti-Histone H3 Ac Lys9 / Ac Lys14 antibody, H3.3B, H3 histone, family 3A, H3.3AH3F3H3F3B, histone H3.3, MGC87783, MGC87782, H3K9ac/K14ac

Purification: Anti-Histone H3 [ac Lys9, ac Lys14] was affinity purified from monospecific antiserum by immunoaffinity chromatography.

Stérilité: Sterile filtered

Immunogène:

Immunogen: Histone H3 [ac Lys9, ac Lys14] affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic acetylated peptide surrounding Lysines 9 and 14 of human Histone H3.2.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry Dilution: 1:200

Application Note: Anti-Histone H3 [ac Lys9, ac Lys14] antibody is tested for Western Blot, Dot Blot, and Immunofluorescence. This antibody is useful for Chromatin Immunoprecipitation and Immunocytochemistry. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~15.4 kDa corresponding to Histone H3 protein by Western Blotting in HeLa histone prep lysate or the appropriate cell lysate or extract. Epi-Plus™ antibody production in collaboration with Novus Biologicals.

ChIP Dilution: 2-5 μg/million cells

Western Blot Dilution: 1:500

IF Microscopy Dilution: 1:200

Other: Dot Blot 1 μg/mL

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.63 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 30 % Glycerol

Preservative: 0.05 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Détails sur Histone 3

Antigène: Histone 3 (H3) (Histone H3 (H3))

Autre désignation: Histone H3

Sujet: Background: Acetylation at K9 and K14 of histone H3 is known to be an epigenetic modification associated with active transcription. Under cellular stress conditions, H3K9ac/K14ac levels increase, probably due to the inactivation of the HDA6 histone deacetylase. Particularly, during infections with herpes simplex virus, the acute phase of the infection is demarcated by increased presence of H3K9ac/K14ac associated with transcriptionally active euchromatin. The virus seems to take advantage of the highly transcribed regions of euchromatin for viral replication, increasing the lytic infection. Anti-Histone H3 are ideal for researchers interested in Chromatin Modifiers, Chromatin Research, Histones and Modified Histones, and Epigenetics research.

ID gène: 126961

NCBI Accession: NP_001005464

UniProt: Q71DI3